Visualization of polydnavirus sequences in a parasitoid wasp chromosome.
نویسندگان
چکیده
Polydnaviruses, obligatorily associated with endoparasitoid wasps, are unique in that their segmented genome is composed of multiple double-stranded DNA circles. We present here the first cytological evidence that virus segments are integrated in the wasp genome, obtained by using in situ hybridization of virus probes with viral sequences in the chromosomes of a wasp from the braconid family of hymenopterans.
منابع مشابه
Wasp Polydnavirus Polymorphism
In this paper, we analyze the polymorphisms of parasitoid wasp polydnavirus. The polydnavius genome is normally dormant within the genome of the wasp, but is converted into particles that are injected into the infected host along with the wasp eggs. These particles contain genes which inhibit the immune system of the infected host, and are necessary for the eggs to mature. Within a population, ...
متن کاملMorphological and genomic characterization of the polydnavirus associated with the parasitoid wasp Glyptapanteles indiensis (Hymenoptera: Braconidae).
Glyptapanteles indiensis polydnavirus (GiPDV) is essential for successful parasitization of the larval stage of the lepidopteran Lymantria dispar (gypsy moth) by the endoparasitic wasp Glyptapanteles indiensis. This virus has not been characterized previously. Ultrastructural studies of GiPDV showed that virions had a rod-like or rectangular form and each contained as many as ten nucleocapsids ...
متن کاملExcision of the polydnavirus chromosomal integrated EP1 sequence of the parasitoid wasp Cotesia congregata (Braconidae, Microgastinae) at potential recombinase binding sites.
Cotesia congregata polydnavirus (CcPDV) is essential for successful parasitism of Manduca sexta larvae by the braconid wasp Cotesia congregata. To determine the molecular mechanisms for the vertical transmission of CcPDV in the wasps, we analysed the different forms of the virus sequences containing the gene encoding the early parasitism-specific protein 1 (EP1). By a detailed molecular analysi...
متن کاملQuantitation of a Glyptapanteles indiensis polydnavirus gene expressed in parasitized host, Lymantria dispar, by real-time quantitative RT-PCR.
Glyptapanteles indiensis is a polydnavirus-carrying wasp that parasitizes early instar gypsy moth larvae. During oviposition, the wasp injects calyx fluid containing polydnavirus along with its eggs into the host. Within the host, expression of polydnavirus genes triggers a set of changes in host physiology, which are of critical importance for the survival of the wasp. In the present study, a ...
متن کاملAccidental Genetic Engineers: Horizontal Sequence Transfer from Parasitoid Wasps to Their Lepidopteran Hosts
We show here that 105 regions in two Lepidoptera genomes appear to derive from horizontally transferred wasp DNA. We experimentally verified the presence of two of these sequences in a diverse set of silkworm (Bombyx mori) genomes. We hypothesize that these horizontal transfers are made possible by the unusual strategy many parasitoid wasps employ of injecting hosts with endosymbiotic polydnavi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of virology
دوره 76 11 شماره
صفحات -
تاریخ انتشار 2002